Flocel Home Home | Request Information
Flocel Home About Us Products Technology Contact Us
Neurological Research
Links
 Request Information
Current Projects
4415 Euclid Ave
Cleveland, OH 44103
216.361.5411
Flocel Products

Testing Services

Flocel Inc. provides in-vitro BBB permeability testing on a contract basis. The fee structure is set by the number of compounds to be tested and the complexity of the testing. The testing service is performed in the laboratories in the Lerner Research Building at the Cleveland Clinic Foundation.

Experiments
  1. Short-term Exclusion Assessment:
    1. To mimic acute permeability after IV injection. Plasma vs. brain levels are compared and "P" value is calculated. To compare these values with know permeant or non-permeant compounds, simultaneous injection of 14C Sucrose and/or 3H-Theophylline is usually employed.
    2. Results are comparable to published "P" values for a variety of compounds.
    3. Compounds are injected intraluminally, and concentrations detected intra and extraluminally for 60 minutes at 5-10 minutes intervals.
  2. Acute determination of Active Exclusion:
    1. To evaluate if a low permeability detected as in 1) is due to
      1. Active extrusion
      2. Poor octanol-water partition
    2. Compounds (three observations per compound) are injected extraluminally, and concentrations detected intra and extraluminally for 60 minutes at 5-10 minutes intervals.
    3. If requested, the experiments may be repeated for the same fee in presence of putative extrusion (e.g., PgP) blockers.
  3. Long-term determination of Active Exclusion:
    1. To evaluate if a low permeability detected as in 1) is due to
      1. Active extrusion
      2. Poor octanol-water partition
    2. The experimental design mimics an "IV drip" with plasma concentrations constant for 24-48 hrs.
    3. Compounds (three observations per compound) are injected intraluminally, and concentrations detected intra and extraluminally for up to two days at 1-2 hrs intervals.
    4. If requested, the experiments may be repeated for the same fee in presence of putative extrusion (e.g., PgP) blockers.
  4. Chronic Exposure/Assessment of Long-term Toxicity and Pharmacokinetic Properties
    1. To study the effects of a given compound on BBB integrity
    2. Measurement of trans-endothelial electrical resistance or Psucrose

Contact Us Online or Call For More Information
Copyright © 2008 Flocel
Feedback Contact